10/30/2022 0 Comments Clc sequence viewer ncbi![]() ![]() Inorganic nitrate (NO 3 -) is a predominant N-containing anion absorbed by upland crops, such as B. Therefore, the development of N-efficient cultivars through genetic improvement of crop NUE is a cost-effective and environmental friendly way to reduce excessive N in soils. The average NUE is approximately 35% in China, which results in N surpluses that are detrimental to the environment. To maintain its optimum yield, relatively high amounts of N fertilizer (from 150 to 300 kg N hm -2) are applied to soils, but only 30–50% of the applied N fertilizer is taken up from soil by crops. Oilseed rape ( Brassica napus L.) is a staple oil crop and has a high N requirement. Hence, enhancing plant N use efficiency (NUE) is critical for developing sustainable agriculture. China is the largest N-consuming country worldwide although massive amounts of N are applied annually as fertilizer, crop yields are declining in some areas. Nitrogen (N) is a fundamental non-mineral macronutrient, which is essential for the growth and development of higher plants. National Center for Biotechnology Information NO 3 -, This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: The data underlying the results presented in the study are available within the manuscript and Supporting Information files.įunding: This study was financially supported by the National Key Research and Development Program of China (2017YFD02001YFD0200103), National Natural Science Foundation of China (31801923, 3110152130), Hunan Provincial Recruitment Program of Foreign Experts, National Oilseed Rape Production Technology System of China, “2011 Plan” supported by the Ministry of Education of China and Double First-class Construction Project of Hunan Agricultural University (kxk201801005).Ĭompeting interests: The authors have declared that no competing interests exist. Received: AugAccepted: NovemPublished: December 20, 2018Ĭopyright: © 2018 Liao et al. PLoS ONE 13(12):Įditor: Anil Kumar Singh, ICAR-Indian Institute of Agricultural Biotechnology, INDIA (2018) Genome-scale characterization of the vacuole nitrate transporter Chloride Channel ( CLC) genes and their transcriptional responses to diverse nutrient stresses in allotetraploid rapeseed. Taken together, our results provide comprehensive insights into the vacuolar BnaCLCs and establish baseline information for future studies on BnaCLCs-mediated vacuolar NO 3 - storage and its effect on NUE.Ĭitation: Liao Q, Zhou T, Yao J-y, Han Q-f, Song H-x, Guan C-y, et al. ![]() The BnaCLC members also showed distinct expression patterns under phosphate depletion and cadmium toxicity. CLCa-3 as the core member of the BnaCLC family, and this gene might play a central role in vacuolar NO 3 - transport in crops. Transcriptomics-assisted gene co-expression network analysis identified BnaA7. High-throughput transcriptional profiles and quantitative real-time PCR results showed that BnaCLCs responded differentially to distinct NO 3 - regimes. The most abundant cis-acting regulatory elements in the gene promoters, including DNA-binding One Zinc Finger, W-box, MYB, and GATA-box, might be involved in the transcriptional regulation of BnaCLCs expression. Segmental duplication was the major driving force in the expansion of the BnaCLC gene family. The genome-wide identification of 22 BnaCLC genes experienced strong purifying selection. The decreased NO 3 - concentration in the clca-2 mutant up-regulated the activities of nitrate reductase and glutamine synthetase, contributing to increase N assimilation and higher NUE in Arabidopsis thaliana. In this study, genomic information, high-throughput transcriptional profiles, and gene co-expression analysis were integrated to identify the CLCs ( BnaCLCs) in Brassica napus. Nitrate distribution to the cytoplasm is beneficial for enhancing NO 3 - assimilation and plays an important role in the regulation of nitrogen (N) use efficiency (NUE). The Chloride Channel ( CLC) gene family is reported to be involved in vacuolar nitrate (NO 3 -) transport. ![]()
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